(Spirodela polyrrhiza (L.) schleid)
HPLC分析條件
1. 層析管：Cosmosil 5C18-AR-II (250 × 4.6 mm, 5 μm)
2. 檢測波長：UV 254 nm
3. 流速：1.0 mL/min
4. 管柱溫度：35 ℃
5. 注入量：5 μL
6. 移動相：
|
時間(min)
|
0.1% formic acid (v/v) &
2 mM ammonium formate in acetonitrile
|
0.1% formic acid (v/v) &
2 mM ammonium formate in H2O
|
|
0
|
5
|
95
|
|
10
|
20
|
80
|
|
20
|
20
|
80
|
|
30
|
21
|
79
|
|
40
|
50
|
50
|
|
|
