UPLC分析條件
1. 層析管:Thermo Syncronis C18 Column (100 x 2.1 mm, 1.7 μm)
2. 檢測波長:UV 254 nm
3. 流速:0.4 mL/min
4. 管柱溫度:35 ℃
5. 注入量:1 μL
6. 移動相:
時間(min)
|
0.1% formic acid (v/v) &
2 mM ammonium formate in acetonitrile
|
0.1% formic acid (v/v) &
2 mM ammonium formate in H2O
|
0
|
5
|
95
|
4
|
20
|
80
|
5
|
20
|
80
|
6
|
21
|
79
|
9
|
50
|
50
|
|
|